Nanovesicles from adipose-derived mesenchymal stem cells inhibit T lymphocyte trafficking and ameliorate chronic experimental autoimmune encephalomyelitis.
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| Peptide Synthesis |
. An amount of 0.3 × 106 CD4+ cells were incubated in complete medium (RPMI + 10% FBS + sodium pyruvate 1 mM + penicillin/streptomycin) in 96-well plates with increasing concentration of MOG35–55 peptide (GenScript), in the presence of 1.2 × 106 irradiated splenocytes as antigen presenting cells. In some experiments, cells were stimulated in the pres-ence of 6, 15 or 30 ng/ml ASC-NVs. Aft |
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