| Products/Services Used | Details | Operation |
|---|---|---|
| Recombinant Proteins | Total protein was extracted in RIPA protein extraction buffer, supplemented with protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Protein concentrations were determined by the BCA Protein Assay Kit (Pierce Thermo Scientific, USA); 10μg of protein was used and electrophoresis was performed in denaturing polyacrylamide 4–20% NuPage gel (Invitrogen, Carlsbad, CA, USA) for 60 min at 140 V and 110 mA and transferred to a nitrocellulose membrane using the eBlot Protein Transfer System using the eBlot Transfer Protein system (Genscript, Piscataway, NJ, USA). | Get A Quote |
Airway remodeling is a characteristic of bronchial asthma. The process involves the expression of many genes， such as transforming growth factor-beta (TGF-β)， tissue inhibitors of metalloproteinases (TIMP-1)， MMP and arginase. Human rhinovirus (HRV) is known to cause asthma exacerbations， and viral infections might be involved in the development of airway remodeling. Therefore， the aim of this study was to determine the influence of HRV on the genes involved in airway remodeling and to examine the impact of thymic stromal lymphopoietin (TSLP) and contribution of oxidative stress on airway remodeling in the context of HRV infection. Peripheral blood mononuclear cells， isolated from blood collected f... More
