Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | Three aliquots of the suspension of raw or modified SiNPs were screened for the presence of endotoxin in duplicate, by chromogenic Limulus Amebocyte Lysate Endotoxin Assay Kit, which was commercially available (Genscript, NJ, USA), following the manufacturer’s protocol. | Get A Quote |
A corona is a layer of macromolecules formed on a nanoparticle surface in vivo. It can substantially change the biological identity of nanomaterials and possibly trigger adverse responses from the body tissues. Dissecting the role of the corona in the development of a particular disease may provide profound insights for understanding toxicity of nanomaterials in general. In our present study, we explored the capability of different silica nanoparticles (SiNPs) to induce silicosis in the mouse lung and analyzed the composition of coronas formed on these particles. We found that SiNPs of certain size and surface chemistry could specifically recruit transforming growth factor β1 (TGF-β1) into their corona, w... More