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Gene repression via multiplex gRNA strategy in Y. lipolytica.

Microb. Cell Fact.. 2018;

ZhangJin-Lai，PengYang-Zi，LiuDuo，LiuHong，CaoYing-Xiu，LiBing-Zhi，LiChun，YuanYing

Products/Services Used	Details	Operation
Codon Optimization	Te fragments of dCpf1 (D917A) derived from Francisella novicida U112 (NC_00860 1) [45], Cas9 and dCas9 (D10A and H841A) derived from Streptococcus pyogenes [36], dCas9-KRAB [37], dCpf1-KRAB, sfGFP [60, 61], VioA, VioB, VioE [62], and vector assembled modules of pMCSCen1 [63], PMCS-Multi, JLPC/Nn and JLRC/N-n were codon optimized and synthesized by GenScript (Nanjing China).	Get A Quote

摘要

The oleaginous yeast Yarrowia lipolytica is a promising microbial cell factory due to their biochemical characteristics and native capacity to accumulate lipid-based chemicals. To create heterogenous biosynthesis pathway and manipulate metabolic flux in Y. lipolytica， numerous studies have been done for developing synthetic biology tools for gene regulation. CRISPR interference (CRISPRi)， as an emerging technology， has been applied for specifically repressing genes of interest.