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Architecture of the human mTORC2 core complex.

Elife. 2018;

StuttfeldEdward，AylettChristopher Hs，ImsengStefan，BoehringerDaniel，ScaiolaAlain，SauerEvelyn，HallMichael N，MaierTimm，BanN

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Peptide Synthesis	The beads were washed 4 times in 50 mM bicine pH 8.5, 200 mM NaCl and bound protein was eluted with DYKDDDDK-peptide (0.6 mg/ml; Genscript, Piscataway, USA)	Get A Quote

摘要

The mammalian target of rapamycin (mTOR) is a key protein kinase controlling cellular metabolism and growth. It is part of the two structurally and functionally distinct multiprotein complexes mTORC1 and mTORC2. Dysregulation of mTOR occurs in diabetes， cancer and neurological disease. We report the architecture of human mTORC2 at intermediate resolution， revealing a conserved binding site for accessory proteins on mTOR and explaining the structural basis for the rapamycin insensitivity of the complex.