High-fat diet (HFD)-fed mouse models have been widely used to study early type 2 diabetes. Decreased β-cell glucokinase (GCK) expression has been observed in HFD-induced diabetes. However， owing to its crucial roles in glucose metabolism in the liver and in islet β-cells， the contribution of decreased GCK expression to the development of HFD-induced diabetes is unclear. Here， we employed a β-cell-targeted gene transfer vector and determined the impact of β-cell-specific increase in GCK expression on β-cell function and glucose handling and Overexpression of GCK enhanced glycolytic flux， ATP-sensitive potassium channel activation and membrane depolarization， and increased proliferation in Min6 c... More
High-fat diet (HFD)-fed mouse models have been widely used to study early type 2 diabetes. Decreased β-cell glucokinase (GCK) expression has been observed in HFD-induced diabetes. However， owing to its crucial roles in glucose metabolism in the liver and in islet β-cells， the contribution of decreased GCK expression to the development of HFD-induced diabetes is unclear. Here， we employed a β-cell-targeted gene transfer vector and determined the impact of β-cell-specific increase in GCK expression on β-cell function and glucose handling and Overexpression of GCK enhanced glycolytic flux， ATP-sensitive potassium channel activation and membrane depolarization， and increased proliferation in Min6 cells. β-cell-targeted GCK transduction did not change glucose handling in chow-fed C57BL/6 mice. Although adult mice fed a HFD showed reduced islet GCK expression， impaired glucose tolerance and decreased glucose-stimulated insulin secretion (GSIS)， β-cell-targeted GCK transduction improved glucose tolerance and restored GSIS. Islet perifusion experiments verified restored GSIS in isolated HFD islets by GCK transduction. Thus， our data identify impaired β-cell GCK expression as an underlying mechanism for dysregulated β-cell function and glycemic control in HFD-induced diabetes. Our data also imply an etiological role of GCK in diet-induced diabetes.This article has an associated First Person interview with the first author of the paper.
