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Engineering de novo anthocyanin production in Saccharomyces cerevisiae.

Microb. Cell Fact.. 2018; 
LevissonMark,PatiniosConstantinos,HeinSascha,de GrootPhilip A,DaranJean-Marc,HallRobert D,MartensStefan,BeekwilderJ
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Codon Optimization Te codon optimized genes of A. thaliana F3H (coF3H; Accession number: NP_190692), ANS (coANS; NP_194019), UGT78D2 (co3GT; NP_197207), and G. hybrida DFR (coGhDFR; P51105) were ordered as synthetic genes (Genscript) as promoter, ORF terminator constructs. Get A Quote

摘要

Anthocyanins are polyphenolic pigments which provide pink to blue colours in fruits and flowers. There is an increasing demand for anthocyanins, as food colorants and as health-promoting substances. Plant production of anthocyanins is often seasonal and cannot always meet demand due to low productivity and the complexity of the plant extracts. Therefore, a system of on-demand supply is useful. While a number of other (simpler) plant polyphenols have been successfully produced in the yeast Saccharomyces cerevisiae, production of anthocyanins has not yet been reported.

关键词

Anthocyanin,Flavonoids,Metabolic engineering,Natural products,Pelargonidin,Plant secondary metabolites,Saccharomyces cerevi