Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … continuously twice for 8 minutes with cooling on ice using an ultrasonic processor FS‐250N (20% amplitude; Shanghai Shengxi, China) … The concentra- tions of endotoxin in the stock solution were measured by using ToxinSensor? Endotoxin Assay Kit (GenScript, NJ, USA … | Get A Quote |
We recently synthesized ZnO nanomaterials (denoted as ZnO nanorods [NRs] and Mini-NRs) and suggested that their cytotoxicity could be related with the activation of endoplasmic reticulum (ER) stress apoptosis. However, in a complex biological microenvironment, the ER stress-apoptosis pathway could also be modulated by biological molecules, such as free fatty acids, leading to unpredicted biological effects. In this study, we investigated the combined toxicity of ZnO NRs/Mini-NRs and palmitate (PA) to THP-1 macrophages. PA influenced the zeta potential and solubility of ZnO NRs and ZnO Mini-NRs in water, which indicated a change of colloidal stability. Exposure to ZnO NRs and Mini-NRs dose-dependent ... More