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Proteins, Expression, Isolation and Analysis | … Endotoxin was removed from the purified rMamINTL using ToxinEraser Endotoxin Removal Kit (GenScript, Nanjing, China), and the removal efficiency was measured using Endotoxin Assay Kit (GenScript) according to the manufacturer's protocol … | Get A Quote |
Intelectin, a lectin discovered recently, has been identified in various vertebrate species, such as fish, amphibians, and mammals. In one of our previous studies, the efficient bacteria binding and agglutinating activity of the recombinant Megalobrama amblycephala intelectin protein (rMamINTL) and the enhanced immunopositive localization have been observed in the hepatic macrophage-like cells (kupffer cells) post Aeromonas hydrophila infection. Thus, the present study primarily focuses on the regulatory effects of rMamINTL on M. amblycephala macrophages. This study revealed a prominent LPS-binding activity of rMamINTL and a significantly increased phagocytosis of rMamINTL-treated A. hydrophila by... More