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Fragmentation of the CRISPR-Cas Type I-B signature protein Cas8b.

Biochim Biophys Acta Gen Subj. 2017-11;

RichterHagen, RompfJudith, WiegelJulia, RauKristina, RandauLen

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Codon Optimization	… C. thermocellum cas genes were codon-optimized for E. coli and synthesized and cloned into vector pUC57 by Genscript … For the plaques assays, a synthetic CRISPR array containing four spacers (see Fig. 6) was synthesized by Genscript and cloned into pUC57 …	Get A Quote

摘要

CRISPR arrays are transcribed into long precursor RNA species， which are further processed into mature CRISPR RNAs (crRNAs). Cas proteins utilize these crRNAs， which contain spacer sequences that can be derived from mobile genetic elements， to mediate immunity during a reoccurring virus infection. Type I CRISPR-Cas systems are defined by the presence of different Cascade interference complexes containing large and small subunits that play major roles during target DNA selection.