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Proteins, Expression, Isolation and Analysis | … Endotoxins were removed using ToxinEraser kits (GenScript, Nanjing, China) and levels were reduced to < 0.1 EU/μg using a Limulus Amebocyte Lysate assay (GenScript) according to the manufacturer's instructions. 2.2. Mice and cell lines … | Get A Quote |
Approximately 80% of ovarian cancer (OC) is diagnosed at late stages, and most patients die within 5 years of diagnosis due to recurrence or drug resistance. Novel treatments are required to improve patient survival. Immune therapy against cancer is promising; however, therapeutic vaccination has been limited by the inability of tumor antigens to induce effective immune responses. Chloride intracellular channel 1 (CLIC1) was previously identified as a possible tumor marker for OC. In this study, we constructed a recombinant protein by conjugating the extracellular domain of CLIC1 to the carboxyl terminus of Mycobacterium tuberculosis heat shock protein 70 (MtHsp70). Human dendritic cells (DCs) derived fro... More