| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Novagen) and gel filtration over Superdex200 (GE Healthcare). EBNA‐LP (UniProt code, Q8AZK7) NLS peptide 29‐RRHRSPSPTRGGQEPRRVRRRV‐50 was synthesized by GenScript. Prior to crystallization, ΔIBB Impα1 and the … | Get A Quote |
Epstein-Barr virus EBNA-LP protein is a transcriptional coactivator of EBNA2. Efficient nuclear localization of EBNA-LP is essential for cooperation with EBNA2. Here， we report the crystal structure of the nuclear import adaptor importin-α1 bound to the nuclear localization signal (NLS) of EBNA-LP that shows EBNA-LP residues 44-RRVRRR-49 binding to the major NLS-binding site at the P0-P5 positions. In contrast to previously characterized classical NLSs that invariably have a basic residue [either lysine (in the vast majority of cases) or arginine] at the P2 position， the EBNA-LP NLS is unique in that it has valine at the P2 position. The loss of the critical P2 lysine (or arginine) is compensated by argini... More
