| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | … All proteins were treated with Endotoxin affinity Resin (Genscript, USA) to remove the endotoxin before followed assays, the purified proteins were shown to have negligible endotoxin (< 10 EU/mg) by an LAL Chromogenic endotoxin quantization assay (Genscript, USA). 2.3 … | Get A Quote |
IL-23/STAT3 signaling pathway is a key process in Th17 cell differentiation， and Th17 cells are closely related to the development of autoimmune diseases. We previously designed and prepared rhIL23R-CHR protein to antagonize endogenous IL-23， showing effectiveness in the treatment of experimental autoimmune encephalomyelitis (EAE) in mice. To further elucidate the mechanism of action， mouse lncRNA microarray was used to screen expression profiles of lncRNAs， and a particular lncRNA， 1700040D17Rik was found to down-regulate in EAE model and its expression was significantly increased after the treatment by rhIL23R-CHR. The function of 1700040D17Rik was revealed to associate with the differentiation of T... More
