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Proteins, Expression, Isolation and Analysis | … The Oscillatoria sp. LPS endotoxin activity was quantified using Toxinsensor chromogenic endotoxin assay kit (Genscript, Piscataway, NJ), according to the manufacturer's instructions, which uses E. coli LPS as a reference standard … | Get A Quote |
Cyanobacteria ("blue-green algae"), such as Oscillatoria sp., are a ubiquitous group of bacteria found in freshwater systems worldwide that are linked to illness and in some cases, death among humans and animals. Exposure to cyanobacteria occurs via ingestion of contaminated water or food-products. Exposure of the gut to these bacteria also exposes their toxins, such as lipopolysaccharide (LPS), to B cells in the gut associated lymphoid tissue. However, the effect of Oscillatoria sp. LPS on B cell activation is unknown. To test the hypothesis that Oscillatoria sp. LPS exposure to murine B cells would result in B cell activation, murine B cells were incubated in the absence or presence of Oscillato... More