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Proteins, Expression, Isolation and Analysis | … a 0.22 μm filter [18]. No endotoxin was detected in AGEs preparations using endotoxin assay kit from Genscript (Piscataway, NJ, USA). Differentiation of 3T3-L1 preadipocytes. 3T3-L1 preadipocytes (from Bioresource Collection … | Get A Quote |
Aging is characterized by mild hyperglycemia and accumulation of advanced glycation end products (AGEs). Effects of chronic exposure to hyperglycemia or AGEs on the adipogenic differentiation of 3T3-L1 preadipocytes remain unclear. We examined the chronic effect of AGEs and high glucose on the differentiation of 3T3-L1 cells by culturing 3T3-L1 cells in the presence of AGEs or 25 mM glucose for 1 month. Chronic incubation of 3T3-L1 cells with AGEs or high glucose blocked their differentiation into mature adipocytes as evidenced by reduced levels of adipocyte markers such as accumulated oil droplets, GPDH, aP2, adiponectin and of adipogenesis regulators PPARγ and C/EBPα. Levels or activities of Src, PD... More