Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | … HA2 was designed and purified as reported previously.20 The endotoxin level from the 3 £ M2e-HA2 in the final vaccine formulation was »3.57 EU/mL, measured using a ToxinSensorTM chromogenic limulus amebocyte lysate endotoxin assay kit from GenScript (Pis- cataway … | Get A Quote |
Some insoluble aluminum salts are commonly used in injectable vaccines as adjuvants to accelerate, prolong, or enhance the antigen-specific immune responses. Data from previous studies testing the nasal mucosal vaccine adjuvant activity of aluminum salts are conflicting. The present study is designed to further assess the feasibility of using aluminum salts in injectable vaccines as nasal mucosal vaccine adjuvants. Using Alhydrogel®, the international scientific standard of aluminum (oxy)hydroxide gels, and ovalbumin or 3 × M2e-HA2, a synthetic influenza virus fusion protein, as antigens, we showed in a mouse model that when dosed intranasally Alhydrogel® enables antigens adsorbed on it to ind... More