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B cell epitope of human cytomegalovirus phosphoprotein 65 (HCMV pp65) induced anti-dsDNA antibody in BALB/c mice.

Arthritis Res. Ther.. 2019-03;

HoHsiehAo, WangChin Man, WuYeong-Jian Jan, ChenAlbert, ChangMing-I, ChenJi

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Peptide Synthesis	… Synthetic peptides. For all synthetic peptides, the purity of the peptide was >95%, per the peptide manufacturer (GenScript, NJ, USA). The preparation of peptides followed the manufacturer's instructions (20 μg/μl), with storage at -80 °C prior to use …	Get A Quote

摘要

HCMV phosphoprotein 65 (HCMVpp65) is a putative immunogen that acts as an accelerator， inducing autoantibody and exacerbating autoimmune response in susceptible animals. The immunity to pp65 instigates autoimmunity， suggesting that pp65 contains crucial B cell epitope(s) for the development of nephritis. This study narrowed down the target epitope to pp65 for immunization of BALB/c mice and mapping of B cell epitope.