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Development & standardization of an in-house IgM indirect ELISA for the detection of parvovirus B19 infections.

Indian J. Med. Res.. 2017-09;

VadivelKumaran, RamamurthyMageshbabu, SankarSathish, JainAmita, SrikanthPadma, GhoshAsit Ranjan, NandagopalBalaji, NairAravindan, SridharanGop

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Peptide Synthesis	… epitopes. The peptide: FSPAASSCHNSSGKEA (length=16 amino acid) was synthesized commercially from GenScript Biotech Corporation, Piscataway, NJ, USA. The assay was similar to previously published protocol 6 . The …	Get A Quote

摘要

Parvovirus B19 infections occur worldwide; the infection is acquired early in childhood but could occur later. B19 is reported to cause infection in childhood febrile illnesses， and arthropathies in adults and children and in end-stage renal disease (ESRD) seen in adults. This study was designed to develop an in-house IgM indirect ELISA for serological screening among patients and controls， and to compare ELISA results with those of nested polymerase chain reaction (nPCR) assay.