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Proteins, Expression, Isolation and Analysis | … described (34). Double stain assay for apoptosis determination.A double stain apoptosis detection kit (Hoechst 33342/PI) (Genscript, Piscataway, NJ, USA) and an inverted confocal microscope (Leica DMI 4000B) were used. The … | Get A Quote |
Free-living amoebae of the genus are the causal agents of a sight-threatening ulceration of the cornea called keratitis, as well as the rare but usually fatal disease granulomatous amoebic encephalitis. Although there are many therapeutic options for the treatment of infections, they are generally lengthy and/or have limited efficacy. For the best clinical outcome, treatments should target both the trophozoite and the cyst stages, as cysts are known to confer resistance to treatment. In this study, we document the activities of caffeine and maslinic acid against both the trophozoite and the cyst stages of three clinical strains of These drugs were chosen because they are reported to inhibit glycog... More