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Proteomic analysis of the gamma human papillomavirus type 197 E6 and E7 associated cellular proteins

Virology. 2017-01;

GraceMiranda, Munger

Products/Services Used	Details	Operation
Codon Optimization	… KCMF1 ubiquitin ligases. 2. Materials and methods. 2.1. Plasmids, antibodies and cell lines. Codon-optimized versions of HPV197 E6 and E7 were custom synthesized by Genscript, Piscataway, NJ. (Table S1). Amino (N) and …	Get A Quote
Bacterial Expression System		Get A Quote

摘要

Gamma HPV197 was the most frequently identified HPV when human skin cancer specimens were analyzed by deep sequencing (Arroyo Muhr et al.， Int. J. Cancer 136: 2546-55， 2015). To gain insight into the biological activities of HPV197， we investigated the cellular interactomes of HPV197 E6 and E7. HPV197 E6 protein interacts with a broad spectrum of cellular LXXLL domain proteins， including UBE3A and MAML1. HPV197 E6 also binds and inhibits the TP53 tumor suppressor and interacts with the CCR4-NOT ubiquitin ligase and deadenylation complex. Despite lacking a canonical retinoblastoma (RB1) tumor suppressor binding site， HPV197 E7 binds RB1 and activates E2F transcription. Hence， HPV197 E6 and E7 protein... More