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Antiproliferative factor (APF) binds specifically to sites within the cytoskeleton-associated protein 4 (CKAP4) extracellular domain

BMC Biochem.. 2017-09;

ChavdaBurzin, LingJun, MajernickThomas, PlaneySonia

Products/Services Used	Details	Operation
Peptide Synthesis	… synthesized by Peptides International (Louisville, KY). Control APF peptide (TVPAAVVVA) at 95% purity was synthesized by GenScript. Generation of CKAP4 recombinant proteins. CKAP4 deletion mutant constructs consisting of …	Get A Quote
Bacterial Expression System		Get A Quote

摘要

Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis-a chronic， painful bladder disease. APF inhibits the proliferation of normal bladder epithelial cells and cancer cells in vitro， presumably by binding to its cellular receptor， cytoskeleton associated-protein 4 (CKAP4); however， the biophysical interaction of APF with CKAP4 has not been characterized previously. In this study， we used surface plasmon resonance (SPR) to explore the binding kinetics of the interaction of APF and as-APF (a desialylated APF analogue with full activity) to CKAP4.