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Catalog Antibody | … GGT variants fused to a 6xHis-tag were detected using a primary mouse anti-His Tag antibody (GenScript, Piscataway, NJ, USA) and fluorescence labeled secondary IRDye 800CW Goat anti-Mouse IgG or IRDye 680RD Goat anti-Mouse IgG antibodies (LI-COR, Lincoln, NE … | Get A Quote |
Semipermeable membranes of cells frequently pose an obstacle in metabolic engineering by limiting uptake of substrates, intermediates, or xenobiotics. Previous attempts to overcome this barrier relied on the promiscuous nature of peptide transport systems, but often suffered from low versatility or chemical instability. Here, we present an alternative strategy to transport cargo molecules across the inner membrane of Escherichia coli based on chemical synthesis of a stable cargo-peptide vector construct, transport through the peptide import system, and efficient intracellular release of the cargo by the promiscuous enzyme γ-glutamyl transferase (GGT). Retaining the otherwise periplasmic GGT in the ... More