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Proteins, Expression, Isolation and Analysis | … Jejunum was sectioned to 5 μ thickness. Jejunum sections were tested for apoptotic changes in their villi using the TUNEL Apoptosis detection kit (GenScript, USA). The test is based on detection of fragmented DNA in the nucleus during apoptosis … | Get A Quote |
Oral administration of berberine chloride to mice induced an obvious enhancement in jejunal health status as expressed by the significant reduction of apoptotic cells within the intestinal villi from 15.5 to 8.3 apoptotic cell/10 VCU. In addition, jejunal antioxidant biomarkers were significantly improved as revealed by the increase in the activities of catalase and glutathione peroxidase enzymes with a concurrent increase in reduced glutathione levels and total antioxidant capacity. Also, it was associated with a significant decrease in oxidative damage biomarkers of hydrogen peroxides, malondialdehyde, nitrite/nitrate, inducible nitric oxide synthase and protein carbonyl content. Moreover, BBR tr... More