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MRE11 stability is regulated by CK2-dependent interaction with R2TP complex.

Oncogene. 2017-08;

von MorgenP, BurdovaK, FlowerT G, O'ReillyN J, BoultonS J, SmerdonS J, MacurekL, HořejŬDepartment of Cancer Cell Biology, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic., Department of Cancer Cell Biology, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic., Structural Biology of DNA-damage Signalling Laboratory, The Francis Crick Institute, London, UK., Peptide Chemistry, The Francis Crick Institute, London, UK., DSB Repair Metabolism Laboratory, The Francis Crick Institute, London, UK., Structural Biology of DNA-damage Signalling Laboratory, The Francis Crick Institute, London, UK., Department of Cancer Cell Biology, Institute of Molecular Genetics of the ASCR, Prague, Czech Republic., Department of Cancer Cell Biology, Institute of Molecular Genetics of the ASCR, Prague, Czech Repub

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Proteins, Expression, Isolation and Analysis	… GST or GST PIH1D1 WT were bound to glutathione sepharose beads (GenScript, Piscataway, NJ, USA) for 2 h at 4 °C, washed with TBS-Tween (150 mM NaCl, 3 mM KCl, 25 mM Tris-HCl (pH 7.2), 10% glycerol) and incubated with 90μl of eluted FLAG MRE11 WT or FLAG …	Get A Quote