| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … 33-mer) and the isotopically labelled peptide LQLQP*FPQPQLPYPQPQLPYPQPQLPYPQ*PQ* P*F (*33-mer) with *P: L-[ 13 C 5 ][ 15 N]-proline and *F: L-[ 13 C 9 ][ 15 N]-phenylalanine, were purchased from Genscript (Hongkong, PR China) with a purity of >90 … | Get A Quote |
Coeliac disease (CD) is triggered by the ingestion of gluten proteins from wheat， rye， and barley. The 33-mer peptide from α2-gliadin has frequently been described as the most important CD-immunogenic sequence within gluten. However， from more than 890 published amino acid sequences of α-gliadins， only 19 sequences contain the 33-mer. In order to make a precise assessment of the importance of the 33-mer， it is necessary to elucidate which wheat species and cultivars contain the peptide and at which concentrations. This paper presents the development of a stable isotope dilution assay followed by liquid chromatography tandem mass spectrometry to quantitate the 33-mer in flours of 23 hexaploid modern ... More
