Products/Services Used | Details | Operation |
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Peptide Synthesis | … A complete list of all peptides and sequences may be found in Supplementary data, Table S1. Peptides 43–88 and A–F were ordered from Abbiotec (San Diego, CA); peptides 89–96 were ordered from GenScript (Piscataway, NJ) … | Get A Quote |
The promiscuity of G-protein-coupled receptors (GPCRs) has broad implications in disease, pharmacology and biosensing. Promiscuity is a particularly crucial consideration for protein engineering, where the ability to modulate and model promiscuity is essential for developing desirable proteins. Here, we present methodologies for (i) modifying GPCR promiscuity using directed evolution and (ii) predicting receptor response and identifying important peptide features using quantitative structure-activity relationship models and grouping-exhaustive feature selection. We apply these methodologies to the yeast pheromone receptor Ste2 and its native ligand α-factor. Using directed evolution, we created Ste2 mu... More