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Introducing the ESAT-6 free IGRA， a companion diagnostic for TB vaccines based on ESAT-6

Sci Rep. 2017-04;

RuhwaldMorten, de ThurahLena, KuchakaDavis, ZaherMostafa Rafaat, SalmanAhmed M, Abdel-GhaffarAbdel-Rahman, ShoukryFaten Aly, MichelsenSascha Wilk, SoborgBolette, BlauenfeldtThomas, MpagamaStellah, HoffSøren T, AggerElse Marie, RosenkrandsIda, AagardClaus, KibikiGibson, El-SheikhNabila, AndersenP

Products/Services Used	Details	Operation
Peptide Synthesis	… Plasma was isolated and frozen for later analysis. Synthetic overlapping peptides (20aa length) were produced by Genscript (NJ, USA). ESAT-6 and CFP10 peptides were designed to be identical as the peptides used in the QFT (15–25aa length) 15 …	Get A Quote

摘要

There is a need for an improved vaccine for tuberculosis. ESAT-6 is a cardinal vaccine antigen with unique properties and is included in several vaccine candidates in development. ESAT-6 is also the core antigen in the IFN-γ release assays (IGRA) used to diagnose latent infection， rendering IGRA tests unspecific after vaccination. This challenge has prompted the development of a companion diagnostic for ESAT-6 based vaccines， an ESAT-6 free IGRA. We screened a panel of seven potential new diagnostic antigens not recognized in BCG vaccinated individuals. Three highly recognized antigens EspC， EspF and Rv2348c were identified and combined with CFP10 in an ESAT-6 free antigen cocktail. The cocktail was prep... More