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Study of kallikrein-related peptidase 6 (KLK6) and its complex with α1-antitrypsin in biological fluids

Clin. Chem. Lab. Med.. 2017-08;

KorbakisDimitrios, SoosaipillaiAntoninus, DiamandisEleftheri

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Recombinant Proteins	… Invitrogen), containing 8 mM l-glutamine. Supernatants were collected and purified using a Protein G column, according to the manufacturer's protocol (GenScript, Piscataway NJ, USA). Culture supernatants were diluted two …	Get A Quote

摘要

Human kallikrein-related peptidase 6 (KLK6) is a member of the kallikrein family of serine proteases. KLK6 is synthesized as a preproenzyme， mainly in tissues of the central nervous system (CNS)， and secreted as an inactive precursor. Serum KLK6 is a biomarker of unfavorable prognosis for ovarian cancer， but its sensitivity for early detection is relatively low. Differential glycosylation of KLK6 has been identified in ascites fluid obtained from ovarian cancer patients， suggesting the presence of unique KLK6 isoforms in biological samples.