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Proteins, Expression, Isolation and Analysis | … Columbia) (Imakawa and others 1987). After purification of crude IFNT by HPLC, we confirmed low levels of endotoxin using the LAL Endotoxin Assay Kit (GenScript, Piscataway, NJ) (Hara and others 2014). The activity of IFNT … | Get A Quote |
Type I interferons (IFN), including IFN-beta (IFNB), activate multiple STAT signaling to drive various biological responses. Another type I IFN, IFN-tau (IFNT), secreted by ruminant embryonic trophoblast cells, has multiple functions with low cytotoxicity. Here, we examined the effects of IFNT on human trophoblast cell functions. First, we performed next-generation sequencing and demonstrated that IFNT-dependent changes in the human Sw.71 trophoblast cell line are partly mediated by proinflammatory as well as IFN signaling. Next, we validated candidate genes, and data confirmed that IFNT stimulated interleukin-6 (IL-6) and IL-8 mRNA expression and secretion. However, human IFNB did not affec... More