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Contribution of VMA5 to vacuolar function， stress response， ion homeostasis and autophagy in Candida albicans

Future Microbiol. 2017-10;

ZhangKai, JiaChang, YuQilin, XiaoChenpeng, DongYijie, ZhangMeng, ZhangDan, ZhaoQiang, ZhangBiao, LiMing

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Catalog Antibody	… The protein was analyzed by SDS-PAGE and western blotting. Both GFP–Atg8 and GFP were detected using the GFP-antibody (Genscript, Nanjing, China), and alpha-tubulin was detected as a control. • RNA isolation & quantitative real-time PCR analysis …	Get A Quote

摘要

V-ATPase is a conservative multi-subunit enzyme in eukaryotes and modulates several cellular responses. This study aimed to illustrate the roles of Vma5 in vacuolar function， oxidative stress response， calcium homeostasis， autophagy and virulence.