Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … and Actin (Proteintech). Fecal and urine albumin were determined via ELISA (Bethyl Labs, Montgomery, AL, USA). The endotoxin level was measured with a commercial kit (GenScript, Nanjing, China). Urine creatinine, Na + and … | Get A Quote |
Chronic high-salt diet-associated renal injury is a key risk factor for the development of hypertension. However, the mechanism by which salt triggers kidney damage is poorly understood. Our study investigated how high salt (HS) intake triggers early renal injury by considering the 'gut-kidney axis'. We fed mice 2% NaCl in drinking water continuously for 8 weeks to induce early renal injury. We found that the 'quantitative' and 'qualitative' levels of the intestinal microflora were significantly altered after chronic HS feeding, which indicated the occurrence of enteric dysbiosis. In addition, intestinal immunological gene expression was impaired in mice with HS intake. Gut permeability elevation and ente... More