| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Peptides were synthesized by F-moc solid-phase peptide synthesis from GenScript Biotech (Nanjing, China) with ≥ 95% purity. The sequences of the synthesized peptides were shown in Table 1. Table 1 Sequences of CVP1, TAT peptide and analogs used in the study. No … | Get A Quote |
Cell-penetrating peptide (CPP) is a promising cargo for delivering bioactive molecules. In this study， the N terminus of VP1 from chicken anemia virus， designated as CVP1， was found to carry enriched arginine residues with α-helix. By confocal imaging， flow cytometry and MTT assay， we identified CVP1 as a novel， safe and efficient CPP. CVP1-FITC peptide could entry different types of cells tested with dose dependence， but without cytotoxic effects. Compared with TAT-FITC peptide， the CVP1-FITC peptide showed much higher cell-penetrating activity. Moreover， CVP1 could successfully deliver β-glycosidase， poly (I:C) and plasmid into HCT116 cells. Inhibitors and temperature sensitivity analysi... More
