| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Peptides of predicted T cell epitopes were synthesized and purified by Nanjing Genscript Biotechnology company. The purity of peptides were >95%. The sequences and positions of these peptides were shown in Table 1. Table 1. Sequences and positions of synthstic peptides … | Get A Quote |
Newly emerging tembusu virus (TMUV) is a severe threat to poultry industry and causes huge economic losses. Humoral and cell-mediated immunity are both play vital roles in TMUV infection. Up to now， there has been no report on identification of T cell epitopes of the TMUV. In this work， we identified T cell epitopes within TMUV envelope (E) protein using synthesized peptides predicted in silico. A total of ten peptides could stimulate TMUV-specific T cells in murine ELISPOT and duck lymphocyte proliferation assay. Subsequently， DNA vaccine containing these T cell epitopes was constructed (pVAX-T) and the expression of multiepitope protein was confirmed by transfection of BHK-21 cells in vitro. Ducks were ... More
