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Peptide Synthesis | … the web version of this article.). 2. Materials and methods. 2.1. Materials. Peptides were synthesized and then purified by high performance liquid chromatography to 95% (GenScript, Piscataway, NJ). Pro-Lys-(Phe-Lys) 5 -Pro … | Get A Quote |
Polymeric nanoparticles (NPs) represent an effective platform for drug delivery systems, albeit with various limitations including low drug loading capacity, cytotoxicity and specificity. NPs composed of the negatively charged Polypeptide, poly gamma glutamic acid (γ-PGA) and a designed amphiphilic and cationic β-sheet Peptide (denoted PoP-NPs) loaded with the drug lonidamine (LND), denoted LND-PoP-NPs were previously used in our lab to successfully target the mitochondria when coated with the peptide (LND-mPoP-NPs). In this study, we improved the drug capacity of the LND-mPoP-NPs in addition to lowering non-specific toxicity associated with the drug deficient mPoP-NPs. LND concentrations in LND-mPo... More