Products/Services Used | Details | Operation |
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Peptide Synthesis | … sequences were provided by Dr. Larry Zeitlin from Mapp Biopharmaceutical, Inc., San Diego, CA), the foot-and-mouth disease virus self-cleaving peptide, and the optimized furin cleavage site 38 were synthesized and codon-optimized for human expression at Genscript, Inc … | Get A Quote |
Vectored delivery of the ZMapp antibody cocktail (c2G4, c4G7, and c13C6) by using recombinant adeno-associated viruses (rAAVs) could be useful for preventive immunization against Ebola virus infections because rAAVs can generate long-term antibody expression. Three rAAVs (serotype 9) encoding chimeric ZMapp antibodies were produced by triple-plasmid transfection up to 10 L-scale in WAVE bioreactors using HEK293 cells grown in suspension/serum-free conditions. Efficacy of AAV-c2G4 via intravenous (i.v.), intramuscular (i.m.), and intranasal (i.n.) routes of administration was evaluated in mice with two different doses of 2.7 × 10 and 13.0 × 10 vector genomes (vg). The best protective efficaci... More