Products/Services Used | Details | Operation |
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Peptide Synthesis | … Peptide Synthesis. All peptides were synthesized as C-terminal free acids and N-terminal free amines with an additional C-terminal GGG-Lys(biotin) for assay immobilization (Genscript, Piscataway NJ, CPC Scientific, Sunnyvale CA, or Abbiotec, San Diego CA). Peptide ELISA … | Get A Quote |
Mimotope peptides selected from combinatorial peptide libraries can be used as capture reagents for immunoassay detection of therapeutic monoclonal antibodies (mAbs). We report the use of phage display libraries to identify peptide ligands (Veritopes) that bind natalizumab, a therapeutic mAb indicated for use in multiple sclerosis. PKNPSKF is identified as a novel natalizumab-binding motif, and peptides containing this motif demonstrated utility as capture reagents in enzyme-linked immunosorbent assays (ELISAs). A peptide containing the identified motif was shown to be competitive with the natural ligand (α4-integrin) and a neutralizing anti-idiotype antibody for natalizumab binding, indicating that Veri... More