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Proteins, Expression, Isolation and Analysis | … 7.4). The lysate was centrifuged at 10,000g for 10 min and analyzed by SDS-PAGE. rMdeiger was purified using Ni-NTA Resin (GenScript, Nanjing, Jiangsu, China) according to the supplier's instructions. Protein concentrations … | Get A Quote |
In mammals, the TNF family is important inflammatory cytokines. Eiger, the invertebrate ortholog of TNF identified firstly in Drosophila, has been implicated in immune response with an unknown molecular mechanism. The present work reports a novel eiger like gene (Mdeiger) from Musca domestica. Mdeiger was significantly up-regulated upon challenge with either Escherichia coli or Staphylococcus aureus. Silencing Mdeiger led to higher mortalities of larvae post either E. coli or S. aureus infection, enhanced the expressions of attacin and diptericin, but blocked the induction of ceropin and muscin, and inhibited the activation of phenoloxidase following bacterial challenge. Meanwhile, the expression ... More