| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Synthesis | … Materials and methods. Peptide synthesis. All synthesized peptides were purchased from GenScript (Piscataway, NJ, USA) or A&PEP (Chungbuk, Korea). All peptides were purified by rapid reverse phase-HPLC to homogeneity (ie, > 90%) and evaluated by mass spectrometry … | Get A Quote |
Many anticancer drugs target epidermal growth factor receptors to inhibit receptor tyrosine kinases and tumor growth. Here， we show that an ErbB2-targeting pronecrotic peptide (KWSY:MTD) selectively kills tumor cells expressing ErbB2 in vitro. An antibody against ErbB2 inhibits KWSY:MTD-induced cell death. KWSY:MTD causes membrane permeability which allows propidium iodide entry into the cytosol and the release of HMGB1 into the media， indicative of necrosis. Mitochondrial swelling occurs in response to KWSY:MTD. Moreover， in vivo analysis using a mouse model shows that KWSY:MTD partially suppressed growth in tumor tissue bearing ErbB2-expressing cells， but did not have obvious toxicity in mouse liver o... More
