Products/Services Used | Details | Operation |
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Peptide Synthesis | … Biotinylation tags were engineered onto the C terminus of each protein. Peptides Peptide MG73 (sequence: KNRLTIELEVRT) derived from human LAMA2 (aa 2784–2795), and scrambled peptide MG73s (sequence: RTLEVINT- KLER) were synthesized by GenScript … | Get A Quote |
Canonical Ag-dependent TCR signaling relies on activation of the src-family tyrosine kinase LCK. However, staphylococcal superantigens can trigger TCR signaling by activating an alternative pathway that is independent of LCK and utilizes a Gα11-containing G protein-coupled receptor (GPCR) leading to PLCβ activation. The molecules linking the superantigen to GPCR signaling are unknown. Using the ligand-receptor capture technology LRC-TriCEPS, we identified LAMA2, the α2 subunit of the extracellular matrix protein laminin, as the coreceptor for staphylococcal superantigens. Complementary binding assays (ELISA, pull-downs, and surface plasmon resonance) provided direct evidence of the interaction be... More