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Peptide Synthesis | … were generated from bone marrow-derived precur- sors, as previously described [7]. DCs were cultured with com- plete RPMI medium containing 50 μM CQ, 100 ng/mL LPS from Escherichia coli O111:B4 (Sigma-Aldrich) and 10 μg/mL MOG35–55 peptide (GenScript) for 18 h … | Get A Quote |
MS and EAE are T cell-driven autoimmune diseases of the CNS where IL-17-producing Th17 cells promote damage and are pathogenic. Conversely, tolerogenic DCs induce Treg cells and suppress Th17 cells. Chloroquine (CQ) suppresses EAE through the modulation of DCs by unknown mechanisms. Here, we show that STAT 1 is necessary for CQ-induced tolerogenic DCs (tolDCs) to efficiently suppress EAE. We observed that CQ induces phosphorylation of STAT1 in DCs in vivo and in vitro. Genetic blockage of STAT1 abrogated the suppressive activity of CQ-treated DCs. Opposed to its WT counterparts, CQ-treated STAT1 BMDCs were unable to suppress Th17 cells and increased EAE severity. Our findings show that STAT1 is a major ... More