Products/Services Used | Details | Operation |
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Peptide Synthesis | … The nucleotide sequence corresponding to the mature Pla a 2 allergen (signal peptides were removed, which cannot be excised by cell recognition and they may affect the proper folding of proteins.) was synthesized by GenScript (Nanjing, China) and was sub-cloned into a … | Get A Quote |
Platanus acerifolia is one of the major sources of outdoor allergens to humans, and can induce allergic asthma, rhinitis, dermatitis and other allergic diseases. Pla a 2 is a polygalacturonase and represents the major allergen identified in P. acerifolia pollen. The aim of the present study was to express and purify Pla a 2, and to predict B and T cell epitopes of Pla a 2. The gene encoding Pla a 2 was cloned into the pET28a vector and subsequently transfected into ArcticExpress™ (DE3) Escherichia coli cells; purified Pla a 2 was analyzed by western blot analysis. The results of the present study revealed that the Pla a 2 allergen has the ability to bind immunoglobulin E within the sera of patien... More