Products/Services Used | Details | Operation |
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Peptide Synthesis | ...two BMV 1a peptides corresponding to amino acids 388 to 422 (helix A) and 415 to 453 (helix B), respectively, were chemically synthesized by GenScript. Dulbecco’s phosphate-buffered... | Get A Quote |
Positive-strand RNA viruses replicate their genomes in membrane-bounded cytoplasmic complexes. We show that endoplasmic reticulum (ER)-linked genomic RNA replication by brome mosaic virus (BMV), a well-studied member of the alphavirus superfamily, depends on the ER luminal thiol oxidase ERO1. We further show that BMV RNA replication protein 1a, a key protein for the formation and function of vesicular BMV RNA replication compartments on ER membranes, permeabilizes these membranes to release oxidizing potential from the ER lumen. Conserved amphipathic sequences in 1a are sufficient to permeabilize liposomes, and mutations in these sequences simultaneously block membrane permeabilization, formation of a disulfide... More