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Catalog Antibody | ... Relative quantification was carried out using the 2-ΔΔCT method. All the primers were chemically syn- thesized by Genscript Biotech (Nanjing, China). Detection of cell proliferation activity Cells (3 × 103/well) were planted into 96-well plates and cultured overnight. ... | Get A Quote |
Long non-coding RNA (lncRNA) H19 was reported to be aberrantly expressed and implicated in non-small cell lung cancer (NSCLC). However, the regulation of NSCLC progression by H19 was not well understood. In this study, we found that the expression level of H19 was obviously increased, but miR-138 was decreased in NSCLC tissues and NSCLC cells including A549, H1299 and H460 cells. Upregulation of H19 promotes cell proliferation ability in A549 and H460 cells, while downregulation of H19 did the opposite. H19 represses miR138 expression and H19 expression level was negatively correlated with miR-138 expression level in NSCLC tissues. Moreover, overexpression of mutant H19 (Mut-H19) has no effect on miR-138 expres... More