PRO AND ANTIAPOPTOTIC EFFECT OF 3, 3-DIINDOLYLMETHANE ON BISPHENOL - A INDUCED APOPTOSIS IN RAT MAMMARY GLANDS AND OVARY.

The purpose of the present study was to investigate the apoptotic effect of DIM on BPA treated mammary and ovary tissues of female Sprague-Dawley rats. Apoptotic markers were analyzed in rat mammary tissue epithelial cells and ovary after chronic administration of BPA (10 μg/kg/bw) alone and along with DIM (5mg/ kg/bw). Immunohistochemical analysis of mammary tissue shows the over expression of Bcl-2 and decreased expression of Bax and western blot analysis shows the decreased expression of cytochrome c, caspases 3, 9 and 8 whereas over expression of Bax and decreased expression of Bcl-2 was observed in ovary. Further, oral administration of DIM in alternative days to BPA treated rats for the period of 12 weeks show a significant decrease in antiapoptotic proteins and increase in proapoptotic proteins in mammary tissues and increase in antiapoptotic proteins and decrease in proapoptotic proteins in ovary. The results of our study demonstrate that DIM has dual effect on BPA induced apoptosis, it promotes apoptosis in mammary tissue on the other hand inhibits apoptosis in ovary. Hence, BPA induced estrogen disruptive apoptosis is overcome by the administration of DIM.