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Proteins, Expression, Isolation and Analysis | ... Total RNA was extracted and reverse transcribed into cDNA, which was then amplified by PCR Master Mix kit (Fermentas) with the primers (Genscript, Nanjing, China) listed as follows: Bag3 (rat), 5′-GAC TGC TCA TCC TCG TCC TC-3' (forward), 5′-GGA TCA CTT GAA TGG ... | Get A Quote |
Many studies reveal that BAG3 plays a critical role in the regulation of protein degradation via macroautophagy. However, it remains unknown whether BAG3 affects the quality control of α-synuclein (SNCA), a Parkinson's disease-related protein. In this study, we demonstrated the increases of BAG3 expression in the ventral midbrain of SNCAA53T transgenic mice and also in MG132-treated PC12 cells overexpressing wild-type SNCA (SNCAWT-PC12). Moreover, we showed that BAG3 overexpression was sufficient to enhance the autophagy activity while knockdown of Bag3 reduced it in SNCAWT-PC12 cells. Immunoprecipitation revealed that BAG3 interacted with heat shock protein 70 and sequestosome 1. The immunostaining also showe... More