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Proteins, Expression, Isolation and Analysis | ... containing 500 mM imidazole). Wash, flow-through and eluted fractions were collected on ice. The fractions with high GST activity were pooled and analyzed by 4-20% (w/v) gradient SDS-PAGE (genscript). The Ni-NTA pooled … | Get A Quote |
The Arabian camel, Camelus dromedarius is naturally adapted to extreme desert climate and has evolved protective mechanisms to limit oxidative stress. The mitochondrial kappa class glutathione transferase enzyme is a member of GST supergene family that represents an important enzyme group in cellular Phase II detoxification machinery and is involved in the protection against oxidative stress and xenobiotics. In the present study, C. dromedarius kappa class glutathione transferase (CdGSTK1-1) was cloned, expressed in E. coli BL21, purified and its structural, thermodynamic and unfolding pathway was investigated. The results showed that CdGSTK1-1 has unique trimeric structure, exhibits low thermostability and a c... More