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Proteins, Expression, Isolation and Analysis | ... 20% gel. The gel was electrotransfered onto NC membranes. After the protein was transferred to membrane, GenScript's ONE-HOUR WesternTM Kit was used for western analysis. 2.12. Immunohistochemistry. Female antennae … | Get A Quote |
Ionotropic receptors (IRs) mainly detect the acids and amines having great importance in many insect species, representing an ancient olfactory receptor family in insects. In the present work, we performed RNAseq of Microplitis mediator antennae and identified seventeen IRs. Full-length MmedIRs were cloned and sequenced. Phylogenetic analysis of the Hymenoptera IRs revealed that ten MmedIR genes encoded "antennal IRs" and seven encoded "divergent IRs". Among the IR25a orthologous groups, two genes, MmedIR25a.1 and MmedIR25a.2, were found in M. mediator. Gene structure analysis of MmedIR25a revealed a tandem duplication of IR25a in M. mediator. The tissue distribution and development specific expression of the M... More