Products/Services Used | Details | Operation |
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Peptide Synthesis | ... for flow cytometry. CFSE was obtained from eBioscience. MR1 (anti-mouse CD40L) was purchased from Bio X Cell (West Lebanon, NH). Peptides were synthesized by GenScript (Piscataway, NJ). Cell isolation Spleen was … | Get A Quote |
Induction of proinflammatory T cell immunity is augmented by innate dendritic cell (DC) maturation commonly initiated by TLR signaling. We demonstrate that ligation of TLR3, TLR4, and TLR9 induces murine DC production of complement components and local production of the anaphylatoxin C5a. In vitro, ex vivo, and in vivo analyses show that TLR-induced DC maturation, as assessed by surface phenotype, expression profiling by gene array, and functional ability to stimulate T cell responses, requires autocrine C3a receptor and C5a receptor (C3ar1/C5ar1) signaling. Studies using bone marrow chimeric animals and Foxp3-GFP/ERT2-Cre/dTomato fate-mapping mice show that TLR-initiated DC autocrine C3ar1/C5ar1 signaling caus... More