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Proteins, Expression, Isolation and Analysis | ... The cell cycle was determined using flow cytom- etry. Briefly, the transfected cells were harvest- ed 12 hrs post-radiation with a dose of 8 Gy for the detection of cells in G1, S and G2 stages using the cell cycle analysis kit (Genscript) according to the manufacturer's instructions. ... | Get A Quote |
Radio-resistance becomes a large obstacle for effective cancer treatment. MicroRNAs (miRNAs) play important roles in response to radiation. However, the underlying mechanism of miR-630 on the radio-resistance of human glioma is less elucidated. In this study, we found that miR-630 was downregulated in glioma cell lines after radiation. MiR-630 inhibition enhanced the survival fraction, cell number in S stage and colony formation ability in glioma cells after radiation, while miR-630 overexpression resulted in inverse effects. By detecting the molecular mechanism of miR-630, we validated that CDC14A was a direct target of miR-630 and miR-630 suppressed CDC14A protein level. CDC14A overexpression can attenuate th... More