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Functional analysis of two polygalacturonase genes in Apolygus lucorum associated with eliciting plant injury using RNA interference.

Arch Insect Biochem Physiol.. 2017-04;

Zhang W,Liu B,Lu Y,Liang G.

Products/Services Used	Details	Operation
Peptide Synthesis	... hemocyanin. The peptides were used as antigen to inject rabbits for antibody production. All procedures were done by GenScript Co., Ltd. (Nanjing, China). The change of protein levels were assessed by western blot. Salivary …	Get A Quote

摘要

Salivary enzymes of many piercing-sucking insects lead to host plant injury. The salivary enzymes, polygalacturonase (PGs), act in insect feeding. PG family genes have been cloned from the mirid bug Apolygus lucorum, a pest of cotton and other host crops in China. We investigated the function of two PG genes that are highly expressed in A. lucorum nymphs (PG3-4) and adults (PG3-5), using siRNA injection-based RNA interference (RNAi). Accumulation of mRNA encoding both genes and their cognate proteins was significantly reduced (>60%) in experimental compared control green fluorescent protein (GFP) siRNA-treated mirids at 48 h post injection. Injury levels of cotton buds were also significantly reduced after inje... More